WebtiTect Reverse Transcription Kit (QIAGEN, USA) in ac-cordancewiththemanufacturer’sinstructions.ThecDNA was kept at −20°C until use. Target fragment amplification and recovery When a gene that was related with survival or repro-duction in either cattle tick (Gong et al., 2008), mosquito (Nene et al., 2007) or fruit fly … WebtiTect Reverse Transcription Kit (Qiagen no. 205313). Real-time RT-PCR The reverse transcription product was subjected to two-step, real-time, RT-PCR amplification on a quantitative PCR System (Bio-Rad CFX96) using SYBR Green master mix (SSoFastEvaGreen,Bio-Radno.172-5202;BioRadLaboratories) and commercially available …

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