Ghd7-cfp
WebAug 10, 2015 · A Ghd7–cyan fluorescent protein (CFP) fusion protein was used as a nuclear localization marker, as Ghd7 is a previously reported nuclear protein in rice (Xue et al., 2008). A SNAC3–GFP fusion protein was co-expressed with Ghd7–CFP in rice protoplasts, and the fluorescence signal was observed and captured using confocal microscopy. WebThe nuclei were identied by co-expressing the nuclear marker Ghd7-CFP with both 35S:SlCOL1-GFP and 35S:GFP. Fluorescence images were acquired using a confocal laser scanning microscope (Leica TCS SP2) after incubating the protoplasts at 28 °C for 12 to 16 h. Representative micrographs are shown. Bars, 7.5 μm (up), 25 μm (down)
Ghd7-cfp
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WebOct 30, 2024 · When an unrelated nuclear localization marker tagged by CFP (Ghd7-CFP) was used, 71 ± 3% or 84 ± 8% of transformed cells determined by the marker showed … Web发现spl13-gfp蛋白完全位于细胞核上，这是基于spl13-gfp和ghd7-cfp发射的荧光的完全重叠。 相反，从游离GFP发出的荧光分布在整个细胞中。 基于这些观察结果，我们得出结 …
WebSep 25, 2024 · I actually got 2lbs of it my orginal intent was to test it with my gen5 g17 but the throat is way to short to use the hornady xtp 147gr with that powder. I eventually tried … WebJul 14, 2024 · We hypothesized that Ghd7 is the background determinant affecting the photoperiod sensitivity of Hd1. To confirm this hypothesis, …
WebSep 14, 2024 · The Ghd7 was used as a cell nucleus localization marker [ 40, 41 ]. The results showed that the YFP signals of both 35S::D5RF.1–YFP and 35S::D5RF.2–YFP were mainly observed in the nucleus ( Figure 3 a,b). Figure 3. Subcellular localization assay for D5RF.1 and D5RF.2 proteins. WebJan 7, 2015 · Ghd7.1 encodes a PRR37-like protein that represses flowering by suppressing the expression of the downstream genes Ehd1 and Hd3a under LD, greatly enhancing grain productivity 23. All these...
WebAug 25, 2015 · Representative cells are shown, as imaged by confocal laser scanning microscopy. a, e, and i, Ghd7:CFP localization in the nucleus (blue fluorescence); b, detection in rice protoplasts of YN:WOX11 and ERF3:YC interaction, shown as yellow signal; f and j, as negative controls with YN:pVYNE (R)/pVYCE (R):YC and YN: pVYNE …
WebMay 28, 2014 · VirD5 is another A. tumefaciens virulence effector protein and conserved in all strains of A. tumefaciens and A. rhizogenes. It consists of 833 amino acids and contains three putative nuclear... can duckweeds remove ammoniaWebMar 1, 2011 · Ghd7, which encodes a CCT-domain-containing transcription factor, was reported to regulate rice grain yield, plant height, and heading date. The natural variation … fish tank at targetWebOur analysis revealed a regulatory network that may provide an approximation to the genetic constitution of the optimal heterosis observed in this hybrid. In this network, Ghd7 works … fish tank at pets at homeWebJan 1, 2013 · It was confirmed that Ghd7 is negatively regulated by Hd17 and Ef7, the same genes with OsELF3, by recent publications ( Matsubara et al., 2012; Saito et al., 2012 ). However, how the Hd17 / Ef7 affects circadian clock genes and further disrupts the photoperiod pathway was not well elucidated. candu energy strikeWebGhd7 was used as a nuclear localization marker. from publication: GmFLD, a soybean homolog of the autonomous pathway gene FLOWERING LOCUS D, promotes flowering … candu energy inc mississaugaWebMar 14, 2024 · GHD7, a nuclear protein, was fused to cyan fluorescent protein (CFP) and co-expressed with OsMFT2-GFP in rice protoplasts. OsMFT2-GFP was co-localized with the GHD7-CFP in the nucleus (Figure 2b ), indicating that OsMFT2 is a nuclear protein. Knock-out and overexpressing OsMFT2 altered ABA sensitivity in seed germination and post … candu crush soda en wiondows no se conectafish tank at wallmart 10 galons