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WebCo-IP is a classic method for studying protein interactions based on the specific interaction between antibodies and antigens. It effectively determines the physiological interaction of two proteins in intact cells. WebPellet again and resuspend in coIP buffer to initial volume (second wash). Pellet cells again and resuspend in coIP buffer to initial volume (third and final wash). Combine the aliquots. Store at 4° for up to 2 days. (Staph A pellets are difficult to resuspend, so it is convenient to split the cells between a number of tubes.
Coip his
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WebApr 11, 2024 · Original Concentration: 200ug/mL. Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive. Labeling. (May be suitable for use in other assays to be determined by the end user.) Predicted isoelectric point: 6.2. Predicted Molecular Mass: 50.3kDa. Accurate Molecular Mass: 50kDa as determined by SDS-PAGE reducing … http://www.proteinguru.com/protocols/IP%20guide2.pdf
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